› 2wzf

X-ray diffraction
2.1Å resolution

Legionella pneumophila glucosyltransferase crystal structure

Released:
DOI: 10.2210/pdb2wzf/pdb
PDB entry 2wzf coloured by chain, front view.
PDB entry 2wzf coloured by chain, side view.
PDB entry 2wzf coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase.
Hurtado-Guerrero R, Zusman T, Pathak S, Ibrahim AF, Shepherd S, Prescott A, Segal G, van Aalten DM
Biochem J 426 281-92 (2010)
PMID: 20030628

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase Lgt1 Chain: A
Molecule details ›
Chain: A
Length: 525 amino acids
Theoretical weight: 59.74 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZVS2 (Residues: 1-525; Coverage: 100%)
Gene name: lpg1368
Sequence domains: Glycosyltransferase family 88
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand UDP 1 x UDP
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 50.999Å b: 104.641Å c: 53.261Å
α: 90° β: 101.6° γ: 90°
R-values:
R R work R free
0.189 0.188 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
4 more

2 mentions without citation

Molecular mechanism of elongation factor 1A inhibition by a Legionella pneumophila glycosyltransferase.
Hurtado-Guerrero et al. (2010)
1 more