› 2wyf

X-ray diffraction
2.4Å resolution

Crystal structure of PA-IL lectin complexed with aGal12bGal-O-Met at 2.4 A resolution

Released:
DOI: 10.2210/pdb2wyf/pdb
PDB entry 2wyf coloured by chain, front view.
PDB entry 2wyf coloured by chain, side view.
PDB entry 2wyf coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Role of water molecules in structure and energetics of Pseudomonas aeruginosa lectin I interacting with disaccharides.
Nurisso A, Blanchard B, Audfray A, Rydner L, Oscarson S, Varrot A, Imberty A
J Biol Chem 285 20316-27 (2010)
PMID: 20410292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
PA-I galactophilic lectin Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 121 amino acids
Theoretical weight: 12.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05097 (Residues: 2-122; Coverage: 99%)
Gene names: PA2570, lecA, pa1L
Sequence domains: PA-IL-like protein
Structure domains: Galactose-binding lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: GLA, MBG
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand GLA 2 x GLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 49.885Å b: 99.788Å c: 91.318Å
α: 90° β: 100.77° γ: 90°
R-values:
R R work R free
0.17 0.166 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
1 more

1 mention without citation

Role of water molecules in structure and energetics of Pseudomonas aeruginosa lectin I interacting with disaccharides.
Nurisso et al. (2010)