› 2wy8

X-ray diffraction
1.7Å resolution

Staphylococcus aureus complement subversion protein Sbi-IV in complex with complement fragment C3d

Released:
DOI: 10.2210/pdb2wy8/pdb
PDB entry 2wy8 coloured by chain, front view.
PDB entry 2wy8 coloured by chain, side view.
PDB entry 2wy8 coloured by chain, top view.
Source organisms:
Primary publication:
A structural basis for Staphylococcal complement subversion: X-ray structure of the complement-binding domain of Staphylococcus aureus protein Sbi in complex with ligand C3d.
Clark EA, Crennell S, Upadhyay A, Zozulya AV, Mackay JD, Svergun DI, Bagby S, van den Elsen JM
Mol Immunol 48 452-62 (2011)
PMID: 21055811

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133850 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Complement C3d fragment Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 34.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01024 (Residues: 996-1303; Coverage: 19%)
Gene names: C3, CPAMD1
Sequence domains: A-macroglobulin TED domain
Structure domains: Glycosyltransferase
IGG-BINDING PROTEIN Chain: Q
Molecule details ›
Chain: Q
Length: 80 amino acids
Theoretical weight: 9.26 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
Structure domains: Sbi, C3 binding domain IV

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 53.43Å b: 81.01Å c: 87.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Regulation of humoral immunity by complement.
Carroll et al. (2012)
10 other articles

5 mentions without citation

Complement component C3: A structural perspective and potential therapeutic implications.
Geisbrecht et al. (2022)
4 more