2wpt Summary



The structure was published by Meenan, N.A., Sharma, A., Fleishman, S.J., et al., Moore, G.R., Baker, D., and Kleanthous, C., in 2010 in a paper entitled "The Structural and Energetic Basis for High Selectivity in a High-Affinity Protein-Protein Interaction." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.78 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely COLICIN-E2 IMMUNITY PROTEIN and COLICIN-E9.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A COLICIN-E2 IMMUNITY PROTEIN P04482 (1-86) (IMM2_ECOLX)search Escherichia colisearch 100% 86 95%
B COLICIN-E9 P09883 (450-582) (CEA9_ECOLX)search Escherichia colisearch < 90% 134 84%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P04482 (1 - 86) COLICIN-E2 IMMUNITY PROTEIN Escherichia coli
P09883 (450 - 582) COLICIN-E9 Escherichia coli

Chain Structural classification (CATH) Sequence family (Pfam)
A (P04482) Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain Asearch PF01320: Colicin immunity protein / pyocin immunity proteinsearch
B Colicin e7 immunity protein. Chain Bsearch DNase/tRNase domain of colicin-like bacteriocinsearch

Chain ID Biological process (GO) Molecular function (GO)
A (P04482) bacteriocin immunitysearch toxic substance bindingsearch
B (P09883) pathogenesissearch cytolysissearch response to bacteriumsearch endonuclease activitysearch receptor bindingsearch

Chain InterPro annotation
A Colicin immunity protein/pyocin immunity proteinsearch Colicin/pyocin immunity protein domainsearch
B Pyocin S killer proteinsearch HNH nucleasesearch Colicin/pyocin, DNase domainsearch