2wpt Summary

pdbe.org/2wpt
spacer

THE CRYSTAL STRUCTURE OF IM2 IN COMPLEX WITH COLICIN E9 DNASE

The structure was published by Meenan, N.A., Sharma, A., Fleishman, S.J., et al., Moore, G.R., Baker, D., and Kleanthous, C., in 2010 in a paper entitled "The Structural and Energetic Basis for High Selectivity in a High-Affinity Protein-Protein Interaction." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.78 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely COLICIN-E2 IMMUNITY PROTEIN and COLICIN-E9.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A COLICIN-E2 IMMUNITY PROTEIN P04482 (1-86) (IMM2_ECOLX)search Escherichia colisearch 100% 86 95%
B COLICIN-E9 P09883 (450-582) (CEA9_ECOLX)search Escherichia colisearch < 90% 134 84%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P04482 (1 - 86) COLICIN-E2 IMMUNITY PROTEIN Escherichia coli
P09883 (450 - 582) COLICIN-E9 Escherichia coli

Chain Structural classification (CATH) Sequence family (Pfam)
A (P04482) Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain Asearch PF01320: Colicin immunity protein / pyocin immunity proteinsearch
B Colicin e7 immunity protein. Chain Bsearch DNase/tRNase domain of colicin-like bacteriocinsearch

Chain ID Molecular function (GO) Biological process (GO)
A (P04482) toxic substance bindingsearch bacteriocin immunitysearch
B (P09883) endonuclease activitysearch receptor bindingsearch pathogenesissearch cytolysissearch response to bacteriumsearch

Chain InterPro annotation
A Colicin immunity protein/pyocin immunity proteinsearch Colicin/pyocin immunity protein domainsearch
B Pyocin S killer proteinsearch HNH nucleasesearch Colicin/pyocin, DNase domainsearch