2wih Summary



The structure was published by Brasca, M.G., Amboldi, N., Ballinari, D., et al., Vianello, P., Vulpetti, A., and Ciomei, M., in 2009 in a paper entitled "Identification of N,1,4,4-Tetramethyl-8-{[4-(4-Methylpiperazin-1-Yl)Phenyl]Amino}-4,5-Dihydro-1H-Pyrazolo[4,3-H]Quinazoline-3-Carboxamide (Pha-848125), a Potent, Orally Available Cyclin Dependent Kinase Inhibitor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 2009.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CELL DIVISION PROTEIN KINASE 2 and CYCLIN-A2.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 309 97%
C CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 309 97%
B CYCLIN-A2 P20248 (173-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 265 96%
D CYCLIN-A2 P20248 (173-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 265 96%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) CELL DIVISION PROTEIN KINASE 2 Homo sapiens
P20248 (173 - 432) CYCLIN-A2 Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, C (P24941) protein phosphorylationsearch positive regulation of cell proliferationsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch cellular response to DNA damage stimulussearch positive regulation of transcription, DNA-templatedsearch DNA repairsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch DNA replicationsearch cellular response to nitric oxidesearch blood coagulationsearch G1/S transition of mitotic cell cyclesearch potassium ion transportsearch meiotic nuclear divisionsearch phosphorylationsearch centrosome duplicationsearch cell cyclesearch meiotic cell cyclesearch histone phosphorylationsearch G2/M transition of mitotic cell cyclesearch Ras protein signal transductionsearch mitotic cell cyclesearch mitotic nuclear divisionsearch regulation of gene silencingsearch mitotic G1 DNA damage checkpointsearch positive regulation of DNA-dependent DNA replication initiationsearch cell divisionsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch ATP bindingsearch protein bindingsearch protein serine/threonine kinase activitysearch cyclin-dependent protein serine/threonine kinase activitysearch transferase activitysearch cyclin bindingsearch kinase activitysearch histone kinase activitysearch protein kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch metal ion bindingsearch nucleotide bindingsearch nucleoplasmsearch cytosolsearch centrosomesearch chromosome, telomeric regionsearch cytoplasmsearch cyclin-dependent protein kinase holoenzyme complexsearch Y chromosomesearch endosomesearch X chromosomesearch condensed chromosomesearch cytoskeletonsearch transcription factor complexsearch microtubule organizing centersearch nucleussearch Cajal bodysearch
B, D (P20248) regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of cell cyclesearch regulation of G2/M transition of mitotic cell cyclesearch protein kinase bindingsearch nucleussearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/F/Osearch Cyclin A, chordatessearch