› 2wei

X-ray diffraction
1.65Å resolution

Crystal structure of the kinase domain of Cryptosporidium parvum calcium dependent protein kinase in complex with 3-MB-PP1

Released:
DOI: 10.2210/pdb2wei/pdb
PDB entry 2wei coloured by chain, front view.
PDB entry 2wei coloured by chain, side view.
PDB entry 2wei coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
The Cryptosporidium parvum kinome.
Artz JD, Wernimont AK, Allali-Hassani A, Zhao Y, Amani M, Lin YH, Senisterra G, Wasney GA, Fedorov O, King O, Roos A, Lunin VV, Qiu W, Finerty P, Hutchinson A, Chau I, von Delft F, MacKenzie F, Lew J, Kozieradzki I, Vedadi M, Schapira M, Zhang C, Shokat K, Heightman T, Hui R
BMC Genomics 12 478 (2011)
PMID: 21962082

Function and Biology Details

Reaction catalysed: 
ATP + D-xylulose = ADP + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-domain protein kinase 1, putative Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 33.45 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3FQ16 (Residues: 70-338; Coverage: 50%)
Gene name: cgd3_920
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand VGG 1 x VGG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 68.9Å b: 68.9Å c: 130.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Cryptosporidium pathogenicity and virulence.
Bouzid et al. (2013)
3 more

9 mentions without citation

ZAP-70: an essential kinase in T-cell signaling.
Wang et al. (2010)
8 more