› 2wa2

X-ray diffraction
1.8Å resolution

Structure of the methyltransferase domain from Modoc Virus, a Flavivirus with No Known Vector (NKV)

Released:
DOI: 10.2210/pdb2wa2/pdb
PDB entry 2wa2 coloured by chain, front view.
PDB entry 2wa2 coloured by chain, side view.
PDB entry 2wa2 coloured by chain, top view.
Source organism: Modoc virus
Primary publication:
Structure of the methyltransferase domain from the Modoc virus, a flavivirus with no known vector.
Jansson AM, Jakobsson E, Johansson P, Lantez V, Coutard B, de Lamballerie X, Unge T, Jones TA
Acta Crystallogr D Biol Crystallogr 65 796-803 (2009)
PMID: 19622863

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NON-STRUCTURAL PROTEIN 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 30.81 KDa
Source organism: Modoc virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8QL64 (Residues: 2474-2744; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand SAM 2 x SAM
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 52.53Å b: 62.242Å c: 160.016Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.235
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The dengue virus NS5 protein as a target for drug discovery.
Lim et al. (2015)
3 more

5 mentions without citation

Flavivirus: From Structure to Therapeutics Development.
Zhao et al. (2021)
4 more