› 2w46

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 14.58 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q51815 (Residues: 155-298; Coverage: 25%)
Gene name: xynD
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 28.216Å b: 46.135Å c: 49.006Å
α: 71.61° β: 89.78° γ: 82.19°
R-values:
R R work R free
0.141 0.137 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

15 review citations

Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates.
Fontes et al. (2010)
14 more

1 mention without citation

Structural and Functional Characterization of a Ruminal β-Glycosidase Defines a Novel Subfamily of Glycoside Hydrolase Family 3 with Permuted Domain Topology.
Ramírez-Escudero et al. (2016)