› 2vxo

X-ray diffraction
2.5Å resolution

Human GMP synthetase in complex with XMP

Released:
DOI: 10.2210/pdb2vxo/pdb
PDB entry 2vxo coloured by chain, front view.
PDB entry 2vxo coloured by chain, side view.
PDB entry 2vxo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Substrate specificity and oligomerization of human GMP synthetase.
Welin M, Lehtiö L, Johansson A, Flodin S, Nyman T, Trésaugues L, Hammarström M, Gräslund S, Nordlund P
J Mol Biol 425 4323-33 (2013)
PMID: 23816837

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GMP synthase [glutamine-hydrolyzing] Chains: A, B
Molecule details ›
Chains: A, B
Length: 697 amino acids
Theoretical weight: 77.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49915 (Residues: 20-693; Coverage: 97%)
Gene name: GMPS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand XMP 2 x XMP
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 98.52Å b: 123.42Å c: 127.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Metabolic channeling: predictions, deductions, and evidence.
Pareek et al. (2021)
4 more

12 mentions without citation

Development of Novel Anti-Leishmanials: The Case for Structure-Based Approaches.
Soni et al. (2022)
11 more