› 2vwb

X-ray diffraction
3.05Å resolution

Structure of the archaeal Kae1-Bud32 fusion protein MJ1130: a model for the eukaryotic EKC-KEOPS subcomplex involved in transcription and telomere homeostasis.

Released:
DOI: 10.2210/pdb2vwb/pdb
PDB entry 2vwb coloured by chain, front view.
PDB entry 2vwb coloured by chain, side view.
PDB entry 2vwb coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex.
Hecker A, Lopreiato R, Graille M, Collinet B, Forterre P, Libri D, van Tilbeurgh H
EMBO J 27 2340-51 (2008)
PMID: 19172740

Function and Biology Details

Reactions catalysed: 
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 535 amino acids
Theoretical weight: 60.66 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58530 (Residues: 1-535; Coverage: 100%)
Gene name: MJ1130
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 147.418Å b: 148.948Å c: 65.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.301
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Protein Ser/Thr/Tyr phosphorylation in the Archaea.
Kennelly PJ. (2014)
4 more

8 mentions without citation

The structural and functional workings of KEOPS.
Beenstock et al. (2021)
7 more