2vss Summary

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WILD-TYPE HYDROXYCINNAMOYL-COA HYDRATASE LYASE IN COMPLEX WITH ACETYL-COA AND VANILLIN

The structure was published by Bennett, J.P., Bertin, L.M., Moulton, B., et al., Brzozowski, A.M., Walton, N.J., and Grogan, G., in 2008 in a paper entitled "A Ternary Complex of Hydroxycinnamoyl-Coa Hydratase-Lyase (Hchl) with Acetyl-Coa and Vanillin Gives Insights Into Substrate Specificity and Mechanism." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.22 Å and deposited in 2008.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterohexamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 89%
B P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 89%
C P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 89%
D P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 89%
E P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 87%
F P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE O69762 (1-276) (HCHL_PSEFL)search Pseudomonas fluorescenssearch 100% 276 89%


This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
O69762 (1 - 276) P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE Pseudomonas fluorescens

Chain Sequence family (Pfam)
A, B, C, D (O69762) PF00378: Enoyl-CoA hydratase/isomerase familysearch
E (O69762) PF00378: Enoyl-CoA hydratase/isomerase familysearch
F (O69762) PF00378: Enoyl-CoA hydratase/isomerase familysearch

Chain ID Molecular function (GO) Biological process (GO)
A, B, C, D (O69762) lyase activitysearch vanillin synthase activitysearch trans-feruloyl-CoA hydratase activitysearch catalytic activitysearch metabolic processsearch
E (O69762) catalytic activitysearch lyase activitysearch vanillin synthase activitysearch trans-feruloyl-CoA hydratase activitysearch metabolic processsearch
F (O69762) catalytic activitysearch vanillin synthase activitysearch lyase activitysearch trans-feruloyl-CoA hydratase activitysearch metabolic processsearch

Chain InterPro annotation
A, B, C, D Crotonase superfamilysearch Enoyl-CoA hydratase/isomerase, conserved sitesearch ClpP/crotonase-like domainsearch
E Crotonase superfamilysearch Enoyl-CoA hydratase/isomerase, conserved sitesearch ClpP/crotonase-like domainsearch
F Crotonase superfamilysearch Enoyl-CoA hydratase/isomerase, conserved sitesearch ClpP/crotonase-like domainsearch