› 2vrh

Electron Microscopy
19Å resolution

Structure of the E. coli trigger factor bound to a translating ribosome

Released:
DOI: 10.2210/pdb2vrh/pdb
PDB entry 2vrh coloured by chain, front view.
PDB entry 2vrh coloured by chain, side view.
PDB entry 2vrh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular mechanism and structure of Trigger Factor bound to the translating ribosome.
Merz F, Boehringer D, Schaffitzel C, Preissler S, Hoffmann A, Maier T, Rutkowska A, Lozza J, Ban N, Bukau B, Deuerling E
EMBO J 27 1622-32 (2008)
PMID: 18497744
Related structures: EMD-1499

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-141618 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Trigger factor Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A850 (Residues: 1-432; Coverage: 100%)
Gene names: JW0426, b0436, tig
Sequence domains:
Large ribosomal subunit protein uL23 Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.22 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ0 (Residues: 1-100; Coverage: 100%)
Gene names: JW3280, b3318, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: C
Molecule details ›
Chain: C
Length: 103 amino acids
Theoretical weight: 11.21 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60624 (Residues: 2-104; Coverage: 99%)
Gene names: JW3271, b3309, rplX
Sequence domains:
Large ribosomal subunit protein uL29 Chain: D
Molecule details ›
Chain: D
Length: 63 amino acids
Theoretical weight: 7.29 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-63; Coverage: 100%)
Gene names: JW3274, b3312, rpmC
Sequence domains: Ribosomal L29 protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
Resolution: 19Å
Relevant EMDB volumes: EMD-1499
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Converging concepts of protein folding in vitro and in vivo.
Hartl et al. (2009)
29 more

4 mentions without citation

Optimized fragmentation schemes and data analysis strategies for proteome-wide cross-link identification.
Liu et al. (2017)
3 more