2vrh Summary

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Structure of the E. coli trigger factor bound to a translating ribosome

The structure was published by Merz, F., Boehringer, D., Schaffitzel, C., et al., Ban, N., Bukau, B., and Deuerling, E., in 2008 in a paper entitled "Molecular Mechanism and Structure of Trigger Factor Bound to the Translating Ribosome." (abstract).

The structure was determined using Electron microscopy at a resolution of 19.0 Å and deposited in 2008.

The experimental data on which the structure is based was deposited separately in EMDB as entry 1499.

This entry contains 4 copies of 4 unique biopolymers (complete list).

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A TRIGGER FACTOR P0A850 (1-432) (TIG_ECOLI)search Escherichia coli K-12search 100% 432 99%
B 50S RIBOSOMAL PROTEIN L23 P0ADZ0 (1-100) (RL23_ECOLI)search Escherichia coli K-12search 92% 100 99%
C 50S RIBOSOMAL PROTEIN L24 P60624 (2-104) (RL24_ECOLI)search Escherichia coli K-12search 95% 103 99%
D 50S RIBOSOMAL PROTEIN L29 P0A7M6 (1-63) (RL29_ECOLI)search Escherichia coli K-12search 95% 63 100%


This entry contains 4 unique UniProt proteins:

UniProt accession Name Organism PDB
P0A850 (1 - 432) TRIGGER FACTOR Escherichia coli
P0ADZ0 (1 - 100) 50S RIBOSOMAL PROTEIN L23 Escherichia coli
P60624 (2 - 104) 50S RIBOSOMAL PROTEIN L24 Escherichia coli
P0A7M6 (1 - 63) 50S RIBOSOMAL PROTEIN L29 Escherichia coli

Chain Structural classification (SCOP) Sequence family (Pfam)
A (P0A850) TF C-terminussearch, Trigger factor ribosome-binding domainsearch, FKBP immunophilin/proline isomerasesearch PF00254: FKBP-type peptidyl-prolyl cis-trans isomerasesearch, PF05697: Bacterial trigger factor protein (TF)search, PF05698: Bacterial trigger factor protein (TF) C-terminussearch
B (P0ADZ0) L23psearch PF00276: Ribosomal protein L23search
C (P60624) Ribosomal proteins L24p and L21esearch PF00467: KOW motifsearch
D (P0A7M6) Ribosomal protein L29 (L29p)search PF00831: Ribosomal L29 proteinsearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (P0A850) protein foldingsearch protein transportsearch cell divisionsearch 'de novo' cotranslational protein foldingsearch protein peptidyl-prolyl isomerizationsearch response to heatsearch cell cyclesearch cytoplasmsearch cytosolsearch membranesearch peptidyl-prolyl cis-trans isomerase activitysearch identical protein bindingsearch isomerase activitysearch protein bindingsearch protein binding involved in protein foldingsearch ribosome bindingsearch
B (P0ADZ0) translationsearch ribosomesearch intracellularsearch ribonucleoprotein complexsearch cytosolic large ribosomal subunitsearch structural constituent of ribosomesearch nucleotide bindingsearch rRNA bindingsearch protein bindingsearch RNA bindingsearch
C (P60624) translationsearch ribosomesearch ribonucleoprotein complexsearch intracellularsearch cytosolic large ribosomal subunitsearch structural constituent of ribosomesearch RNA bindingsearch rRNA bindingsearch
D (P0A7M6) translationsearch intracellularsearch ribosomesearch ribonucleoprotein complexsearch cytosolic large ribosomal subunitsearch structural constituent of ribosomesearch RNA bindingsearch rRNA bindingsearch

Chain InterPro annotation
A Peptidyl-prolyl cis-trans isomerase, FKBP-type, domainsearch Trigger factorsearch Trigger factor, C-terminal domainsearch Trigger factor, ribosome-binding, bacterialsearch Trigger factor/SurA domainsearch
B Ribosomal protein L23/L25, conserved sitesearch Nucleotide-binding alpha-beta plait domainsearch Ribosomal protein L23/L15e core domainsearch Ribosomal protein L25/L23search
C Ribosomal protein L24search KOWsearch Ribosomal protein L24/L26, conserved sitesearch Translation protein SH3-like domainsearch Ribosomal protein L2 domain 2search
D Ribosomal protein L29search Ribosomal protein L29, conserved sitesearch