› 2vpx

X-ray diffraction
3.1Å resolution

Polysulfide reductase with bound quinone (UQ1)

Released:
DOI: 10.2210/pdb2vpx/pdb
PDB entry 2vpx coloured by chain, front view.
PDB entry 2vpx coloured by chain, side view.
PDB entry 2vpx coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Molecular mechanism of energy conservation in polysulfide respiration.
Jormakka M, Yokoyama K, Yano T, Tamakoshi M, Akimoto S, Shimamura T, Curmi P, Iwata S
Nat Struct Mol Biol 15 730-7 (2008)
PMID: 18536726

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-180887 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
4Fe-4S Mo/W bis-MGD-type domain-containing protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 765 amino acids
Theoretical weight: 86.74 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: Q72LA4 (Residues: 1-765; Coverage: 100%)
Gene name: TT_C0155
Sequence domains:
Structure domains:
4Fe-4S ferredoxin-type domain-containing protein Chains: B, F
Molecule details ›
Chains: B, F
Length: 195 amino acids
Theoretical weight: 21.41 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: Q72LA5 (Residues: 1-195; Coverage: 100%)
Gene names: TT_C0154, nrfC
Sequence domains:
Structure domains: Alpha-Beta Plaits
Hypothetical membrane spanning protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 253 amino acids
Theoretical weight: 27.62 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: Q72LA6 (Residues: 1-253; Coverage: 100%)
Gene name: TT_C0153
Sequence domains: Polysulfide reductase
Structure domains: Formate dehydrogenase/DMSO reductase domain

Ligands and Environments

4 bound ligands:
Ligand SF4 10 x SF4
Ligand MGD 4 x MGD
Ligand MO 2 x MO
Ligand UQ1 2 x UQ1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 114.587Å b: 161.162Å c: 239.647Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.301 0.301 0.314

Quick links

Citations

13 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
12 more

6 mentions without citation

Bacterial Oxidases of the Cytochrome bd Family: Redox Enzymes of Unique Structure, Function, and Utility As Drug Targets.
Borisov et al. (2021)
5 more