spacer
EBI PDBe PDBeView

PDBe Entry: 2vo5 view

STRUCTURAL AND BIOCHEMICAL EVIDENCE FOR A BOAT-LIKE TRANSITION STATE IN BETA-MANNOSIDASES
Summary
Header HYDROLASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.3 Å, R-factor: 17.3%, Free R-factor: 24.7%, Spacegroup: P 1 21 1
Released 01/04/2008, deposition: 08/02/2008, last revision: 24/02/2009
Authors Tailford, L.E.search; Offen, W.A.search; Smith, N.L.search; Dumon, C.search; Moreland, C.search; Gratien, J.search; Heck, M.P.search; Stick, R.V.search; Bleriot, Y.search; Vasella, A.search; Gilbert, H.J.search; Davies, G.J.search
Primary citation Structural and Biochemical Evidence for a Boat-Like Transition State in Beta-Mannosidases.
NAT.CHEM.BIOL.search vol:4, pag:306 (2008) [PubMed ID 18408714 ]search
Keywords GLYCOSIDEsearch, HYDROLASEsearch, MANNOSIDASEsearch, TRANSITION STATE MIMICsearch, LINEAR FREE ENERGY RELATIONSHIPsearch
Organism Bacteroides thetaiotaomicron VPI-5482 226186search(A B)
UniProt Beta-mannosidase Q8AAK6search (A B)
Solvent A, B
Related entries 2vqt, 2vot, 2vl4, 2vqu, 2vo5, 2je8, 2vjx, 2vmf
Polymers
Id Name Type UniProt Residues Observed
A, B BETA-MANNOSIDASE Protein Q8AAK6 (Q8AAK6_BACTN)search
 846 99%
Heterogens
Id Name Ligands
A, B (1R,4R,5R,7R,8R)-2-BENZYL-5-HYDROXYMETHYL-2-AZA-BICYCLO[2.2.2]OCTANE-4,7,8-TRIOL VBZ search
A, B 1,2-ETHANEDIOL EDO search
A, B BROMIDE ION BR search
A, B CHLORIDE ION CL search
spacer