› 2vnj

X-ray diffraction
2.13Å resolution

X-RAY STRUCTURE OF THE FERREDOXIN-NADP(H) REDUCTASE FROM RHODOBACTER CAPSULATUS IN COMPLEX WITH NADP. FORM I AT 2.13 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb2vnj/pdb
PDB entry 2vnj coloured by chain, front view.
PDB entry 2vnj coloured by chain, side view.
PDB entry 2vnj coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
Coenzyme binding and hydride transfer in Rhodobacter capsulatus ferredoxin/flavodoxin NADP(H) oxidoreductase.
Bortolotti A, Pérez-Dorado I, Goñi G, Medina M, Hermoso JA, Carrillo N, Cortez N
Biochim Biophys Acta 1794 199-210 (2009)
PMID: 18973834

Function and Biology Details

Reactions catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Reduced flavodoxin + NADP(+) = oxidized flavodoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin/ferredoxin--NADP reductase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.44 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9L6V3 (Residues: 1-270; Coverage: 100%)
Gene name: fpr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP

Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand HTG 1 x HTG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P3121
Unit cell:
a: 68.72Å b: 68.72Å c: 128.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.237 0.271
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+.
Medina M. (2009)
1 more

2 mentions without citation

Crystal structure of the FAD-containing ferredoxin-NADP+ reductase from the plant pathogen Xanthomonas axonopodis pv. citri.
Tondo et al. (2013)
1 more