› 2vf7

X-ray diffraction
2.3Å resolution

Crystal structure of UvrA2 from Deinococcus radiodurans

Released:
DOI: 10.2210/pdb2vf7/pdb
PDB entry 2vf7 coloured by chain, front view.
PDB entry 2vf7 coloured by chain, side view.
PDB entry 2vf7 coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
Structural and mutational analyses of Deinococcus radiodurans UvrA2 provide insight into DNA binding and damage recognition by UvrAs.
Timmins J, Gordon E, Caria S, Leonard G, Acajjaoui S, Kuo MS, Monchois V, McSweeney S
Structure 17 547-58 (2009)
PMID: 19368888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UvrABC system protein A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 842 amino acids
Theoretical weight: 91.66 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RYW8 (Residues: 81-922; Coverage: 91%)
Gene name: DR_A0188
Sequence domains: UvrA DNA-binding domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 6 x ADP
Ligand ZN 6 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 271.71Å b: 111.67Å c: 103.29Å
α: 90° β: 100.8° γ: 90°
R-values:
R R work R free
0.223 0.219 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Prokaryotic nucleotide excision repair.
Kisker et al. (2013)
7 more

7 mentions without citation

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges.
Timmins et al. (2016)
6 more