2vdu Summary

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STRUCTURE OF TRM8-TRM82, THE YEAST TRNA M7G METHYLATION COMPLEX

The structure was published by Leulliot, N., Chaillet, M., Durand, D., Ulryck, N., Blondeau, K., and Van Tilbeurgh, H., in 2008 in a paper entitled "Structure of the Yeast tRNA M7G Methylation Complex." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.4 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE-ASSOCIATED WD REPEAT PROTEIN TRM82 and TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
B TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE-ASSOCIATED WD REPEAT PROTEIN TRM82 Q03774 (1-444) (TRM82_YEAST)search Saccharomyces cerevisiae S288csearch 100% 450 83%
D TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE-ASSOCIATED WD REPEAT PROTEIN TRM82 Q03774 (1-444) (TRM82_YEAST)search Saccharomyces cerevisiae S288csearch 100% 450 83%
E TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE Q12009 (39-286) (TRMB_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 254 79%
F TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE Q12009 (39-286) (TRMB_YEAST)search Saccharomyces cerevisiae S288csearch < 90% 254 79%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
Q03774 (1 - 444) TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE-ASSOCIATED WD REPEAT PROTEIN TRM82 Saccharomyces cerevisiae
Q12009 (39 - 286) TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE Saccharomyces cerevisiae

Chain Sequence family (Pfam)
B, D (Q03774) PF00400: WD domain, G-beta repeatsearch
E, F Putative methyltransferasesearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
B, D (Q03774) protein bindingsearch tRNA (guanine-N7-)-methyltransferase activitysearch cytosolsearch nucleussearch tRNA methyltransferase complexsearch RNA (guanine-N7)-methylationsearch tRNA methylationsearch tRNA processingsearch
E, F (Q12009) tRNA (guanine-N7-)-methyltransferase activitysearch tRNA modificationsearch

Chain InterPro annotation
B, D WD40 repeatsearch WD40/YVTN repeat-like-containing domainsearch WD40-repeat-containing domainsearch tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit, Trm82search
E, F tRNA (guanine-N-7) methyltransferasesearch tRNA (guanine-N-7) methyltransferase catalytic subunit Trm8, eukaryotesearch S-adenosyl-L-methionine-dependent methyltransferase-likesearch