› 2vad

X-ray diffraction
1.59Å resolution

Monomeric red fluorescent protein, DsRed.M1

Released:
DOI: 10.2210/pdb2vad/pdb
PDB entry 2vad coloured by chain, front view.
PDB entry 2vad coloured by chain, side view.
PDB entry 2vad coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Structural rearrangements near the chromophore influence the maturation speed and brightness of DsRed variants.
Strongin DE, Bevis B, Khuong N, Downing ME, Strack RL, Sundaram K, Glick BS, Keenan RJ
Protein Eng Des Sel 20 525-34 (2007)
PMID: 17962222

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 25.44 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 1-221; Coverage: 98%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand ZN 3 x ZN
Ligand CL 3 x CL
Ligand EDO 2 x EDO
1 modified residue:
Ligand CRQ 1 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 38.892Å b: 62.151Å c: 81.882Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.197
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
28 other articles

5 mentions without citation

BioBits™ Bright: A fluorescent synthetic biology education kit.
Stark et al. (2018)
4 more