› 2v8n

X-ray diffraction
3.6Å resolution

Wild-type Structure of Lactose Permease

Released:
DOI: 10.2210/pdb2v8n/pdb
PDB entry 2v8n coloured by chain, front view.
PDB entry 2v8n coloured by chain, side view.
PDB entry 2v8n coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural determination of wild-type lactose permease.
Guan L, Mirza O, Verner G, Iwata S, Kaback HR
Proc Natl Acad Sci U S A 104 15294-8 (2007)
PMID: 17881559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02920 (Residues: 1-417; Coverage: 100%)
Gene names: JW0334, b0343, lacY
Sequence domains: LacY proton/sugar symporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 101.37Å b: 127.4Å c: 188.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.294 0.294 0.333
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Ins and outs of major facilitator superfamily antiporters.
Law et al. (2008)
22 more

47 mentions without citation

Energy coupling mechanisms of MFS transporters.
Zhang et al. (2015)
46 more