› 2v6f

X-ray diffraction
2.4Å resolution

Structure of Progesterone 5beta-Reductase from Digitalis Lanata

Released:
DOI: 10.2210/pdb2v6f/pdb
PDB entry 2v6f coloured by chain, front view.
PDB entry 2v6f coloured by chain, side view.
PDB entry 2v6f coloured by chain, top view.
Source organism: Digitalis lanata
Primary publication:
The crystal structure of progesterone 5beta-reductase from Digitalis lanata defines a novel class of short chain dehydrogenases/reductases.
Thorn A, Egerer-Sieber C, Jäger CM, Herl V, Müller-Uri F, Kreis W, Muller YA
J Biol Chem 283 17260-9 (2008)
PMID: 18032383

Function and Biology Details

Reaction catalysed: 
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP(+) = cortisone + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxo-Delta(4,5)-steroid 5-beta-reductase Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 41.33 KDa
Source organism: Digitalis lanata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PQJ9 (Residues: 26-389; Coverage: 94%)
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 78.66Å b: 78.66Å c: 180.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.205 0.251
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
3 more

2 mentions without citation

The Vein Patterning 1 (VEP1) gene family laterally spread through an ecological network.
Tarrío et al. (2011)
1 more