› 2v62

X-ray diffraction
1.7Å resolution

Structure of vaccinia-related kinase 2

Released:
DOI: 10.2210/pdb2v62/pdb
PDB entry 2v62 coloured by chain, front view.
PDB entry 2v62 coloured by chain, side view.
PDB entry 2v62 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site.
Scheeff ED, Eswaran J, Bunkoczi G, Knapp S, Manning G
Structure 17 128-38 (2009)
PMID: 19141289

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase VRK2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 39.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86Y07 (Residues: 14-335; Coverage: 63%)
Gene name: VRK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand SIN 1 x SIN
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 70.655Å b: 157.532Å c: 56.288Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
38 more

6 mentions without citation

Emerging roles of the αC-β4 loop in protein kinase structure, function, evolution, and disease.
Yeung et al. (2020)
5 more