› 2v5e

X-ray diffraction
2.35Å resolution

The structure of the GDNF:Coreceptor complex: Insights into RET signalling and heparin binding.

Released:
DOI: 10.2210/pdb2v5e/pdb
PDB entry 2v5e coloured by chain, front view.
PDB entry 2v5e coloured by chain, side view.
PDB entry 2v5e coloured by chain, top view.
Source organisms:
Primary publication:
The structure of the glial cell line-derived neurotrophic factor-coreceptor complex: insights into RET signaling and heparin binding.
Parkash V, Leppänen VM, Virtanen H, Jurvansuu JM, Bespalov MM, Sidorova YA, Runeberg-Roos P, Saarma M, Goldman A
J Biol Chem 283 35164-72 (2008)
PMID: 18845535

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153978 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
GDNF family receptor alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.54 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q62997 (Residues: 150-349; Coverage: 45%)
Gene names: Gdnfra, Gfra1, Retl1, Trnr1
Sequence domains: GDNF/GAS1 domain
Glial cell line-derived neurotrophic factor Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P39905 (Residues: 111-211; Coverage: 53%)
Gene name: GDNF
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

Carbohydrate polymer : NEW Components: GU4, YYJ
Carbohydrate polymer
2 bound ligands:
Ligand EDO 4 x EDO
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 58.9Å b: 75.7Å c: 105.5Å
α: 90° β: 91.9° γ: 90°
R-values:
R R work R free
0.187 0.184 0.237
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Signaling in cell differentiation and morphogenesis.
Basson MA. (2012)
14 more

6 mentions without citation

The structure of the glial cell line-derived neurotrophic factor-coreceptor complex: insights into RET signaling and heparin binding.
Parkash et al. (2008)
5 more