› 2v09

X-ray diffraction
1.8Å resolution

SENS161-164DSSN mutant of Bacillus subtilis Oxalate Decarboxylase OxdC

Released:
DOI: 10.2210/pdb2v09/pdb
PDB entry 2v09 coloured by chain, front view.
PDB entry 2v09 coloured by chain, side view.
PDB entry 2v09 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Oxalate decarboxylase and oxalate oxidase activities can be interchanged with a specificity switch of up to 282,000 by mutating an active site lid.
Burrell MR, Just VJ, Bowater L, Fairhurst SA, Requena L, Lawson DM, Bornemann S
Biochemistry 46 12327-36 (2007)
PMID: 17924657

Function and Biology Details

Reaction catalysed: 
Oxalate = formate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxalate decarboxylase OxdC Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 43.61 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34714 (Residues: 1-385; Coverage: 100%)
Gene names: BSU33240, oxdC, yvrK
Sequence domains: Cupin
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: R32
Unit cell:
a: 154.602Å b: 154.602Å c: 122.957Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.141 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Oxygen activation by mononuclear Mn, Co, and Ni centers in biology and synthetic complexes.
Fiedler et al. (2017)
19 other articles

1 mention without citation

Structural and immunologic characterization of Ara h 1, a major peanut allergen.
Chruszcz et al. (2011)