› 2uzw

X-ray diffraction
2.2Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132597 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 336 amino acids
Theoretical weight: 39.22 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00517 (Residues: 16-351; Coverage: 96%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: Q3SX13 (Residues: 6-25; Coverage: 26%)
Gene name: PKIA

Ligands and Environments

1 bound ligand:
Ligand SS4 1 x SS4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P212121
Unit cell:
a: 72.904Å b: 74.932Å c: 80.243Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.313 0.313 0.328
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Novel inhibitors of AKT: assessment of a different approach targeting the pleckstrin homology domain.
Meuillet EJ. (2011)
2 more

2 mentions without citation

Activation of Aurora-A kinase by protein partner binding and phosphorylation are independent and synergistic.
Dodson et al. (2012)
1 more