› 2uzi

X-ray diffraction
2Å resolution

Crystal structure of HRAS(G12V) - anti-RAS Fv complex

Released:
DOI: 10.2210/pdb2uzi/pdb
PDB entry 2uzi coloured by chain, front view.
PDB entry 2uzi coloured by chain, side view.
PDB entry 2uzi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tumour prevention by a single antibody domain targeting the interaction of signal transduction proteins with RAS.
Tanaka T, Williams RL, Rabbitts TH
EMBO J 26 3250-9 (2007)
PMID: 17568777

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208342 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTI-RAS FV HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 114 amino acids
Theoretical weight: 12.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
ANTI-RAS FV LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 104 amino acids
Theoretical weight: 11.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
GTPase HRas, N-terminally processed Chain: R
Molecule details ›
Chain: R
Length: 166 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 5 x ZN
Ligand GTP 1 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 75.528Å b: 84.632Å c: 62.592Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Therapeutic antibodies: successes, limitations and hopes for the future.
Chames et al. (2009)
29 more

16 mentions without citation

Drugging Ras GTPase: a comprehensive mechanistic and signaling structural view.
Lu et al. (2016)
15 more