2uw8 Summary

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STRUCTURE OF PKA-PKB CHIMERA COMPLEXED WITH 2-(4-CHLORO-PHENYL)-2-PHENYL-ETHYLAMINE

The structure was published by Saxty, G., Woodhead, S.J., Berdini, V., et al., Downham, R., Garrett, M.D., and Carr, R.A., in 2007 in a paper entitled "Identification of Inhibitors of Protein Kinase B Using Fragment-Based Lead Discovery." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.0 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT and CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT P00517 (2-351) (KAPCA_BOVIN)search Bos taurussearch 96% 351 96%
I CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA P61925 (6-25) (IPKA_HUMAN)search Homo sapienssearch < 90% 20 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00517 (2 - 351) CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT Bos taurus
P61925 (6 - 25) CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA HOMO SAPIENS

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00517) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
I cAMP-dependent protein kinase inhibitorsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P00517) protein phosphorylationsearch positive regulation of cell cycle arrestsearch cellular response to parathyroid hormone stimulussearch sperm capacitationsearch regulation of protein processingsearch phosphorylationsearch peptidyl-threonine phosphorylationsearch mesoderm formationsearch regulation of osteoblast differentiationsearch peptidyl-serine phosphorylationsearch cellular response to glucose stimulussearch regulation of synaptic transmissionsearch positive regulation of protein export from nucleussearch protein autophosphorylationsearch regulation of tight junction assemblysearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch neural tube closuresearch regulation of proteasomal protein catabolic processsearch ATP bindingsearch protein kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch protein serine/threonine kinase activitysearch nucleotide bindingsearch protein kinase A regulatory subunit bindingsearch protein bindingsearch cAMP-dependent protein kinase activitysearch protein serine/threonine/tyrosine kinase activitysearch kinase activitysearch transferase activitysearch ubiquitin protein ligase bindingsearch protein kinase bindingsearch cytoplasmsearch ciliary basesearch plasma membranesearch sperm midpiecesearch nucleussearch neuromuscular junctionsearch extracellular vesicular exosomesearch mitochondrionsearch centrosomesearch membranesearch AMP-activated protein kinase complexsearch
I (P61925) negative regulation of protein kinase activitysearch cAMP-dependent protein kinase inhibitor activitysearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
I cAMP-dependent protein kinase inhibitorsearch