2uw3 Summary

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STRUCTURE OF PKA-PKB CHIMERA COMPLEXED WITH 5-METHYL-4-PHENYL-1H-PYRAZOLE

The structure was published by Saxty, G., Woodhead, S.J., Berdini, V., et al., Downham, R., Garrett, M.D., and Carr, R.A., in 2007 in a paper entitled "Identification of Inhibitors of Protein Kinase B Using Fragment-Based Lead Discovery" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.19 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT and CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT P00517 (2-351) (KAPCA_BOVIN)search Bos taurussearch 96% 351 96%
I CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA P61925 (6-25) (IPKA_HUMAN)search Homo sapienssearch < 90% 20 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P00517 (2 - 351) CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT Bos taurus
P61925 (6 - 25) CAMP-DEPENDENT PROTEIN KINASE INHIBITOR ALPHA HOMO SAPIENS

Chain Structural classification (CATH) Sequence family (Pfam)
A (P00517) Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
I cAMP-dependent protein kinase inhibitorsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A (P00517) nucleussearch AMP-activated protein kinase complexsearch centrosomesearch membranesearch mitochondrionsearch plasma membranesearch neuromuscular junctionsearch cytoplasmsearch ciliary basesearch extracellular vesicular exosomesearch sperm midpiecesearch protein kinase activitysearch protein serine/threonine kinase activitysearch protein bindingsearch ATP bindingsearch transferase activity, transferring phosphorus-containing groupssearch transferase activitysearch kinase activitysearch cAMP-dependent protein kinase activitysearch protein kinase A regulatory subunit bindingsearch nucleotide bindingsearch protein kinase bindingsearch protein serine/threonine/tyrosine kinase activitysearch ubiquitin protein ligase bindingsearch sperm capacitationsearch regulation of synaptic transmissionsearch regulation of tight junction assemblysearch peptidyl-serine phosphorylationsearch cellular response to glucose stimulussearch protein phosphorylationsearch mesoderm formationsearch phosphorylationsearch neural tube closuresearch positive regulation of protein export from nucleussearch positive regulation of cell cycle arrestsearch regulation of protein processingsearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch regulation of proteasomal protein catabolic processsearch protein autophosphorylationsearch peptidyl-threonine phosphorylationsearch cellular response to parathyroid hormone stimulussearch regulation of osteoblast differentiationsearch
I (P61925) cAMP-dependent protein kinase inhibitor activitysearch negative regulation of protein kinase activitysearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
I cAMP-dependent protein kinase inhibitorsearch