› 2uvl

X-ray diffraction
1.91Å resolution

Human BIR3 domain of Baculoviral Inhibitor of Apoptosis Repeat- Containing 3 (BIRC3)

Released:
DOI: 10.2210/pdb2uvl/pdb
PDB entry 2uvl coloured by chain, front view.
PDB entry 2uvl coloured by chain, side view.
PDB entry 2uvl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of BIR domains from human NAIP and cIAP2.
Herman MD, Moche M, Flodin S, Welin M, Trésaugues L, Johansson I, Nilsson M, Nordlund P, Nyman T
Acta Crystallogr Sect F Struct Biol Cryst Commun 65 1091-6 (2009)
PMID: 19923725

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Baculoviral IAP repeat-containing protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13489 (Residues: 242-337; Coverage: 16%)
Gene names: API2, BIRC3, MIHC, RNF49
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 49.41Å b: 53.72Å c: 85.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Zinc-rich inhibitor of apoptosis proteins (IAPs) as regulatory factors in the epithelium of normal and inflamed airways.
Roscioli et al. (2013)
7 other articles

9 mentions without citation

Discovery of a potent small-molecule antagonist of inhibitor of apoptosis (IAP) proteins and clinical candidate for the treatment of cancer (GDC-0152).
Flygare et al. (2012)
8 more