› 2udp

X-ray diffraction
1.8Å resolution

UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL

Released:
DOI: 10.2210/pdb2udp/pdb
PDB entry 2udp coloured by chain, front view.
PDB entry 2udp coloured by chain, side view.
PDB entry 2udp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High-resolution X-ray structure of UDP-galactose 4-epimerase complexed with UDP-phenol.
Thoden JB, Frey PA, Holden HM
Protein Sci 5 2149-61 (1996)
PMID: 8931134

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09147 (Residues: 1-338; Coverage: 100%)
Gene names: JW0742, b0759, galD, galE
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand UPP 2 x UPP
Ligand NA 1 x NA
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 76Å b: 78.7Å c: 128.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
2 more