› 2tmd

X-ray diffraction
2.4Å resolution

CORRELATION OF X-RAY DEDUCED AND EXPERIMENTAL AMINO ACID SEQUENCES OF TRIMETHYLAMINE DEHYDROGENASE

Released:
DOI: 10.2210/pdb2tmd/pdb
PDB entry 2tmd coloured by chain, front view.
PDB entry 2tmd coloured by chain, side view.
PDB entry 2tmd coloured by chain, top view.
Source organism: Methylophilus methylotrophus W3A1
Primary publication:
Correlation of x-ray deduced and experimental amino acid sequences of trimethylamine dehydrogenase.
Barber MJ, Neame PJ, Lim LW, White S, Matthews FS
J Biol Chem 267 6611-9 (1992)
PMID: 1551870

Function and Biology Details

Reaction catalysed: 
Trimethylamine + H(2)O + electron-transferring flavoprotein = dimethylamine + formaldehyde + reduced electron-transferring flavoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trimethylamine dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 729 amino acids
Theoretical weight: 81.61 KDa
Source organism: Methylophilus methylotrophus W3A1
Expression system: Not provided
UniProt:
  • Canonical: P16099 (Residues: 2-730; Coverage: 100%)
Gene name: tmd
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
2 bound ligands:
Ligand SF4 2 x SF4
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 147.75Å b: 71.95Å c: 83.82Å
α: 90° β: 97.69° γ: 90°
R-values:
R R work R free
0.154 0.154 not available
Expression system: Not provided

Quick links

Citations

2 review citations

Oxidation of amines by flavoproteins.
Fitzpatrick PF. (2010)
1 more

10 mentions without citation

Role of hydrophobic clusters and long-range contact networks in the folding of (alpha/beta)8 barrel proteins.
Selvaraj et al. (2003)
9 more