Function and Biology

THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA SEGMENTS

Source organism: Escherichia coli
Biochemical function: metal ion binding
Biological process: DNA restriction-modification system
Cellular component: not assigned

EC 3.1.21.4: Type II site-specific deoxyribonuclease

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Systematic name:
-
Alternative Name(s):
  • Type II restriction enzyme

Sequence family

Pfam Protein family (Pfam)
PF09233
Domain description: Restriction endonuclease EcoRV
Occurring in:
  1. Type II restriction enzyme EcoRV
The deposited structure of PDB entry 2rve contains 2 copies of Pfam domain PF09233 (Restriction endonuclease EcoRV) in Type II restriction enzyme EcoRV. Showing 1 copy in chain E [auth A].

InterPro InterPro annotations
IPR015314
Domain description: Restriction endonuclease, type II, EcoRV
Occurring in:
  1. Type II restriction enzyme EcoRV
IPR011335
Domain description: Restriction endonuclease type II-like
Occurring in:
  1. Type II restriction enzyme EcoRV
IPR037057
Domain description: DNA mismatch repair MutH/Type II restriction endonuclease superfamily
Occurring in:
  1. Type II restriction enzyme EcoRV

Structure domain

CATH CATH domain
3.40.600.10
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: ECO RV Endonuclease; Chain A
Homology: DNA mismatch repair MutH/Restriction endonuclease, type II
Occurring in:
  1. Type II restriction enzyme EcoRV
The deposited structure of PDB entry 2rve contains 2 copies of CATH domain 3.40.600.10 (ECO RV Endonuclease; Chain A) in Type II restriction enzyme EcoRV. Showing 1 copy in chain E [auth A].
SCOP SCOP annotation
52984
Class: Alpha and beta proteins (a/b)
Fold: Restriction endonuclease-like
Superfamily: Restriction endonuclease-like
Occurring in:
  1. Type II restriction enzyme EcoRV
The deposited structure of PDB entry 2rve contains 2 copies of SCOP domain 52984 (Restriction endonuclease EcoRV) in Type II restriction enzyme EcoRV. Showing 1 copy in chain E [auth A].