› 2rl1

X-ray diffraction
2.2Å resolution

Crystal structure of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-acetylglucosamine

Released:
DOI: 10.2210/pdb2rl1/pdb
PDB entry 2rl1 coloured by chain, front view.
PDB entry 2rl1 coloured by chain, side view.
PDB entry 2rl1 coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Crystal structure of UDP-N-acetylglucosamine enolpyruvyl transferase from Haemophilus influenzae in complex with UDP-N-acetylglucosamine and fosfomycin.
Yoon HJ, Lee SJ, Mikami B, Park HJ, Yoo J, Suh SW
Proteins 71 1032-7 (2008)
PMID: 18247346

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 45.24 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45025 (Residues: 1-424; Coverage: 100%)
Gene names: HI_1081, murA, murZ
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand UD1 1 x UD1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: I222
Unit cell:
a: 63.907Å b: 123.377Å c: 127.634Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.248
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural perspective of peptidoglycan biosynthesis and assembly.
Lovering et al. (2012)
3 more

2 mentions without citation

Functional consequence of covalent reaction of phosphoenolpyruvate with UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA).
Zhu et al. (2012)
1 more