› 2rhi

X-ray diffraction
1.66Å resolution

Crystal structure of the 3-MBT domain from human L3MBTL1 in complex with H1.5K27me2 at 1.66 angstrom

Released:
DOI: 10.2210/pdb2rhi/pdb
PDB entry 2rhi coloured by chain, front view.
PDB entry 2rhi coloured by chain, side view.
PDB entry 2rhi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger.
Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ
Mol Cell 28 677-91 (2007)
PMID: 18042461

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147699 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lethal(3)malignant brain tumor-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 39.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y468 (Residues: 265-594; Coverage: 39%)
Gene names: KIAA0681, L3MBT, L3MBTL, L3MBTL1
Sequence domains: mbt repeat
Structure domains: SH3 type barrels.
Histone H1.5 Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 605 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16401 (Residues: 23-27; Coverage: 2%)
Gene names: H1-5, H1F5, HIST1H1B

Ligands and Environments

2 bound ligands:
Ligand PG4 1 x PG4
Ligand PEG 1 x PEG
1 modified residue:
Ligand MLY 1 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 85.864Å b: 93.318Å c: 58.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.201
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

52 review citations

Histones: annotating chromatin.
Campos et al. (2009)
51 more

5 mentions without citation

Readout of epigenetic modifications.
Patel et al. (2013)
4 more