› 2rfq

X-ray diffraction
1.65Å resolution

Crystal structure of 3-HSA hydroxylase from Rhodococcus sp. RHA1

Released:
DOI: 10.2210/pdb2rfq/pdb
PDB entry 2rfq coloured by chain, front view.
PDB entry 2rfq coloured by chain, side view.
PDB entry 2rfq coloured by chain, top view.
Source organism: Rhodococcus jostii RHA1
Entry authors: Chang C, Skarina T, Kagan O, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent monooxygenase, oxygenase subunit HsaA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 394 amino acids
Theoretical weight: 43.59 KDa
Source organism: Rhodococcus jostii RHA1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0S811 (Residues: 1-382; Coverage: 100%)
Gene names: RHA1_ro04539, hsaA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 1PS 13 x 1PS
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 73.283Å b: 76.137Å c: 80.881Å
α: 83.78° β: 75.95° γ: 89.99°
R-values:
R R work R free
0.147 0.146 0.171
Expression system: Escherichia coli

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