2rd0 Summary


Structure of a human p110alpha/p85alpha complex

The structure was published by Huang, C.H., Mandelker, D., Schmidt-Kittler, O., et al., Vogelstein, B., Gabelli, S.B., and Amzel, L.M., in 2007 in a paper entitled "The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.05 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform and Phosphatidylinositol 3-kinase regulatory subunit alpha.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform P42336 (1-1068) (PK3CA_HUMAN)search Homo sapienssearch 100% 1096 90%
B Phosphatidylinositol 3-kinase regulatory subunit alpha P27986 (322-600) (P85A_HUMAN)search Homo sapienssearch < 90% 279 49%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P42336 (1 - 1068) Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Homo sapiens
P27986 (322 - 600) Phosphatidylinositol 3-kinase regulatory subunit alpha Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P42336) Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1search, C2- domain Calcium/lipid binding domainsearch, Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch, Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 4search, Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 5search PF00454: Phosphatidylinositol 3- and 4-kinasesearch, PF00613: Phosphoinositide 3-kinase family, accessory domain (PIK domain)search, PF00792: Phosphoinositide 3-kinase C2search, PF00794: PI3-kinase family, ras-binding domainsearch, PF02192: PI3-kinase family, p85-binding domainsearch
B SH2 domainsearch

Chain ID Biological process (GO) Cellular component (GO) Molecular function (GO)
A (P42336) phosphatidylinositol phosphorylationsearch phosphatidylinositol-mediated signalingsearch cardiac muscle contractionsearch small molecule metabolic processsearch negative regulation of fibroblast apoptotic processsearch phospholipid metabolic processsearch fibroblast growth factor receptor signaling pathwaysearch protein kinase B signalingsearch Fc-epsilon receptor signaling pathwaysearch negative regulation of neuron apoptotic processsearch T cell receptor signaling pathwaysearch platelet activationsearch T cell costimulationsearch epidermal growth factor receptor signaling pathwaysearch regulation of multicellular organism growthsearch glucose metabolic processsearch vasculature developmentsearch positive regulation of protein kinase activitysearch angiogenesissearch insulin receptor signaling pathwaysearch phosphatidylinositol-3-phosphate biosynthetic processsearch leukocyte migrationsearch insulin receptor signaling pathway via phosphatidylinositol 3-kinasesearch regulation of genetic imprintingsearch blood coagulationsearch Fc-gamma receptor signaling pathway involved in phagocytosissearch innate immune responsesearch protein phosphorylationsearch negative regulation of anoikissearch positive regulation of peptidyl-serine phosphorylationsearch endothelial cell migrationsearch hypomethylation of CpG islandsearch phosphorylationsearch phosphatidylinositol biosynthetic processsearch neurotrophin TRK receptor signaling pathwaysearch cytosolsearch plasma membranesearch lamellipodiumsearch 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complexsearch phosphatidylinositol 3-kinase complexsearch kinase activitysearch protein bindingsearch protein kinase activator activitysearch phosphotransferase activity, alcohol group as acceptorsearch ATP bindingsearch 1-phosphatidylinositol-4-phosphate 3-kinase activitysearch transferase activitysearch nucleotide bindingsearch phosphatidylinositol-4,5-bisphosphate 3-kinase activitysearch 1-phosphatidylinositol-3-kinase activitysearch phosphatidylinositol 3-kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch protein serine/threonine kinase activitysearch insulin receptor substrate bindingsearch
B (P27986) phosphatidylinositol 3-kinase complexsearch phosphatidylinositol 3-kinase regulator activitysearch

Chain InterPro annotation
A C2 domainsearch Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domainsearch Phosphatidylinositol 3-/4-kinase, catalytic domainsearch Phosphoinositide 3-kinase, accessory (PIK) domainsearch Phosphatidylinositol 3-kinase, C2 domainsearch Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domainsearch Protein kinase-like domainsearch Phosphatidylinositol Kinasesearch Armadillo-type foldsearch Phosphatidylinositol 3/4-kinase, conserved sitesearch Ubiquitin-related domainsearch
B SH2 domainsearch PI3 kinase, P85 regulatory subunitsearch