2rd0 Summary


Structure of a human p110alpha/p85alpha complex

The structure was published by Huang, C.H., Mandelker, D., Schmidt-Kittler, O., et al., Vogelstein, B., Gabelli, S.B., and Amzel, L.M., in 2007 in a paper entitled "The structure of a human p110alpha/p85alpha complex elucidates the effects of oncogenic PI3Kalpha mutations." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.05 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform and Phosphatidylinositol 3-kinase regulatory subunit alpha.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform P42336 (1-1068) (PK3CA_HUMAN)search Homo sapienssearch 100% 1096 90%
B Phosphatidylinositol 3-kinase regulatory subunit alpha P27986 (322-600) (P85A_HUMAN)search Homo sapienssearch < 90% 279 49%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P42336 (1 - 1068) Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Homo sapiens
P27986 (322 - 600) Phosphatidylinositol 3-kinase regulatory subunit alpha Homo sapiens

Chain Structural classification (CATH) Sequence family (Pfam)
A (P42336) Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1search, C2- domain Calcium/lipid binding domainsearch, Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeatsearch, Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 4search, Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 5search PF00454: Phosphatidylinositol 3- and 4-kinasesearch, PF00613: Phosphoinositide 3-kinase family, accessory domain (PIK domain)search, PF00792: Phosphoinositide 3-kinase C2search, PF00794: PI3-kinase family, ras-binding domainsearch, PF02192: PI3-kinase family, p85-binding domainsearch
B SH2 domainsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A (P42336) transferase activity, transferring phosphorus-containing groupssearch phosphatidylinositol-4,5-bisphosphate 3-kinase activitysearch protein bindingsearch phosphotransferase activity, alcohol group as acceptorsearch insulin receptor substrate bindingsearch kinase activitysearch protein kinase activator activitysearch 1-phosphatidylinositol-4-phosphate 3-kinase activitysearch phosphatidylinositol 3-kinase activitysearch 1-phosphatidylinositol-3-kinase activitysearch ATP bindingsearch transferase activitysearch nucleotide bindingsearch protein serine/threonine kinase activitysearch phosphatidylinositol phosphorylationsearch phosphatidylinositol biosynthetic processsearch neurotrophin TRK receptor signaling pathwaysearch endothelial cell migrationsearch negative regulation of anoikissearch T cell costimulationsearch protein phosphorylationsearch phosphatidylinositol-mediated signalingsearch Fc-epsilon receptor signaling pathwaysearch fibroblast growth factor receptor signaling pathwaysearch protein kinase B signalingsearch phosphatidylinositol-3-phosphate biosynthetic processsearch cardiac muscle contractionsearch phospholipid metabolic processsearch small molecule metabolic processsearch regulation of multicellular organism growthsearch positive regulation of protein kinase activitysearch hypomethylation of CpG islandsearch platelet activationsearch negative regulation of neuron apoptotic processsearch epidermal growth factor receptor signaling pathwaysearch vasculature developmentsearch phosphorylationsearch positive regulation of peptidyl-serine phosphorylationsearch T cell receptor signaling pathwaysearch blood coagulationsearch Fc-gamma receptor signaling pathway involved in phagocytosissearch innate immune responsesearch insulin receptor signaling pathway via phosphatidylinositol 3-kinasesearch insulin receptor signaling pathwaysearch leukocyte migrationsearch negative regulation of fibroblast apoptotic processsearch glucose metabolic processsearch angiogenesissearch regulation of genetic imprintingsearch cytosolsearch lamellipodiumsearch phosphatidylinositol 3-kinase complexsearch plasma membranesearch 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complexsearch
B (P27986) phosphatidylinositol 3-kinase regulator activitysearch phosphatidylinositol 3-kinase complexsearch

Chain InterPro annotation
A C2 domainsearch Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domainsearch Phosphatidylinositol 3-/4-kinase, catalytic domainsearch Phosphoinositide 3-kinase, accessory (PIK) domainsearch Phosphatidylinositol 3-kinase, C2 domainsearch Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domainsearch Protein kinase-like domainsearch Phosphatidylinositol Kinasesearch Armadillo-type foldsearch Phosphatidylinositol 3/4-kinase, conserved sitesearch Ubiquitin-related domainsearch
B SH2 domainsearch PI3 kinase, P85 regulatory subunitsearch