› 2ram

X-ray diffraction
2.4Å resolution

A NOVEL DNA RECOGNITION MODE BY NF-KB P65 HOMODIMER

Released:
DOI: 10.2210/pdb2ram/pdb
PDB entry 2ram coloured by chain, front view.
PDB entry 2ram coloured by chain, side view.
PDB entry 2ram coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A novel DNA recognition mode by the NF-kappa B p65 homodimer.
Chen YQ, Ghosh S, Ghosh G
Nat Struct Biol 5 67-73 (1998)
PMID: 9437432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115830 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcription factor p65 Chains: A, B
Molecule details ›
Chains: A, B
Length: 273 amino acids
Theoretical weight: 31.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04207 (Residues: 19-291; Coverage: 50%)
Gene names: Nfkb3, Rela
Sequence domains:
Structure domains:
DNA (5'-D(*CP*GP*GP*CP*TP*GP*GP*AP*AP*AP*TP*(5IU)P*(5IU)P*CP*CP*AP*GP*CP*CP*G)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.36 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand DTV 2 x DTV
1 modified residue:
Ligand 5IU 4 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 65.81Å b: 80.62Å c: 167.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.308
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

28 review citations

Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity.
Karin et al. (2000)
27 more

33 mentions without citation

Proteasome Biology: Chemistry and Bioengineering Insights.
Račková et al. (2020)
32 more