› 2r36

X-ray diffraction
2Å resolution

Crystal structure of ni human ARG-insulin

Released:
DOI: 10.2210/pdb2r36/pdb
PDB entry 2r36 coloured by chain, front view.
PDB entry 2r36 coloured by chain, side view.
PDB entry 2r36 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Metal induced structural changes observed in hexameric insulin.
Sreekanth R, Pattabhi V, Rajan SS
Int J Biol Macromol 44 29-36 (2009)
PMID: 18977386

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 22 amino acids
Theoretical weight: 2.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 89-110; Coverage: 26%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand NI 4 x NI
Ligand NA 1 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R3
Unit cell:
a: 83.953Å b: 83.953Å c: 40.222Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.221 0.255

Quick links

Citations

1 citations in other articles

Endosomal proteolysis of internalised [ArgA0]-human insulin at neutral pH generates the mature insulin peptide in rat liver in vivo.
Kouach et al. (2009)