› 2qyo

X-ray diffraction
1.95Å resolution

Crystal structure of isoflavone O-methyltransferase homolog in complex with biochanin A and SAH

Released:
DOI: 10.2210/pdb2qyo/pdb
PDB entry 2qyo coloured by chain, front view.
PDB entry 2qyo coloured by chain, side view.
PDB entry 2qyo coloured by chain, top view.
Source organism: Medicago truncatula
Entry authors: Wang C, Yu X-H, Liu C-J

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoflavone-7-O-methyltransferase 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 357 amino acids
Theoretical weight: 40.26 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06YR3 (Residues: 1-357; Coverage: 100%)
Gene names: 11419743, MTR_7g014510, MtrunA17_Chr7g0219141
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
2 bound ligands:
Ligand K 13 x K
Ligand QSO 2 x QSO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 44.54Å b: 82.049Å c: 101.034Å
α: 90° β: 101.94° γ: 90°
R-values:
R R work R free
0.214 0.214 0.245
Expression system: Escherichia coli BL21(DE3)

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