› 2qvh

X-ray diffraction
1.76Å resolution

Crystal structure of O-succinylbenzoate synthase complexed with O-succinyl benzoate (OSB)

Released:
DOI: 10.2210/pdb2qvh/pdb
PDB entry 2qvh coloured by chain, front view.
PDB entry 2qvh coloured by chain, side view.
PDB entry 2qvh coloured by chain, top view.
Source organism: Thermobifida fusca YX
Entry authors: Sugadev R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 35.5 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47Q21 (Residues: 2-317; Coverage: 100%)
Gene names: Tfu_1410, menC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand OSB 2 x OSB
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 44.543Å b: 52.877Å c: 61.449Å
α: 70.53° β: 87.22° γ: 85.56°
R-values:
R R work R free
0.218 0.218 0.251
Expression system: Escherichia coli BL21(DE3)

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