› 2qqu

X-ray diffraction
2.84Å resolution

Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose

Released:
DOI: 10.2210/pdb2qqu/pdb
PDB entry 2qqu coloured by chain, front view.
PDB entry 2qqu coloured by chain, side view.
PDB entry 2qqu coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose.
Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W
J Mol Biol 377 378-85 (2008)
PMID: 18258263

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Beta-fructofuranosidase, insoluble isoenzyme CWINV1 Chain: A
Molecule details ›
Chain: A
Length: 535 amino acids
Theoretical weight: 60.86 KDa
Source organism: Arabidopsis thaliana
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q43866 (Residues: 48-582; Coverage: 96%)
Gene names: At3g13790, BFRUCT1, CWINV1, MMM17.26
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 4 x ZN
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P32
Unit cell:
a: 106.271Å b: 106.271Å c: 50.434Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.194 0.253
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
2 more

4 mentions without citation

Crystal structures of Aspergillus japonicus fructosyltransferase complex with donor/acceptor substrates reveal complete subsites in the active site for catalysis.
Chuankhayan et al. (2010)
3 more