› 2qf2

X-ray diffraction
1.65Å resolution

Rat cytosolic PEPCK in complex with oxaloacetic acid and GDP.

Released:
DOI: 10.2210/pdb2qf2/pdb
PDB entry 2qf2 coloured by chain, front view.
PDB entry 2qf2 coloured by chain, side view.
PDB entry 2qf2 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structures of rat cytosolic PEPCK: insight into the mechanism of phosphorylation and decarboxylation of oxaloacetic acid.
Sullivan SM, Holyoak T
Biochemistry 46 10078-88 (2007)
PMID: 17685635

Function and Biology Details

Reaction catalysed: 
GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] Chains: A, B
Molecule details ›
Chains: A, B
Length: 624 amino acids
Theoretical weight: 69.64 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07379 (Residues: 1-622; Coverage: 100%)
Gene name: Pck1
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN 7 x MN
Ligand NA 2 x NA
Ligand OAA 2 x OAA
Ligand GDP 3 x GDP
Ligand PYR 2 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 63.506Å b: 119.112Å c: 86.71Å
α: 90° β: 107.35° γ: 90°
R-values:
R R work R free
0.206 0.204 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
2 more

8 mentions without citation

Lessons from high-throughput protein crystallization screening: 10 years of practical experience.
Luft et al. (2011)
7 more