2qcs Summary


A complex structure between the Catalytic and Regulatory subunit of Protein Kinase A that represents the inhibited state

The structure was published by Kim, C., Cheng, C.Y., Saldanha, S.A., and Taylor, S.S., in 2007 in a paper entitled "PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2007.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely cAMP-dependent protein kinase, alpha-catalytic subunit and cAMP-dependent protein kinase type I-alpha regulatory subunit.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterodimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A cAMP-dependent protein kinase, alpha-catalytic subunit P05132 (2-351) (KAPCA_MOUSE)search Mus musculussearch 96% 350 96%
B cAMP-dependent protein kinase type I-alpha regulatory subunit P00514 (91-380) (KAP0_BOVIN)search Bos taurussearch 100% 291 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P05132 (2 - 351) cAMP-dependent protein kinase, alpha-catalytic subunit Mus musculus
P00514 (91 - 380) cAMP-dependent protein kinase type I-alpha regulatory subunit Bos taurus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P05132) Protein kinases, catalytic subunitsearch Transferase(Phosphotransferase) domain 1search, Phosphorylase Kinase; domain 1search PF00069: Protein kinase domainsearch
B (P00514) PF00027: Cyclic nucleotide-binding domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P05132) protein phosphorylationsearch regulation of proteasomal protein catabolic processsearch regulation of synaptic transmissionsearch protein autophosphorylationsearch regulation of protein processingsearch sperm capacitationsearch peptidyl-serine phosphorylationsearch peptidyl-threonine phosphorylationsearch mesoderm formationsearch positive regulation of protein export from nucleussearch regulation of osteoblast differentiationsearch phosphorylationsearch neural tube closuresearch regulation of tight junction assemblysearch cellular response to glucose stimulussearch positive regulation of cell cycle arrestsearch cellular response to parathyroid hormone stimulussearch negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterningsearch ATP bindingsearch protein kinase activitysearch protein serine/threonine kinase activitysearch transferase activity, transferring phosphorus-containing groupssearch nucleotide bindingsearch protein kinase bindingsearch protein bindingsearch cAMP-dependent protein kinase activitysearch transferase activitysearch ubiquitin protein ligase bindingsearch kinase activitysearch protein kinase A regulatory subunit bindingsearch protein serine/threonine/tyrosine kinase activitysearch ciliary basesearch cytosolsearch membranesearch cytoplasmsearch neuromuscular junctionsearch centrosomesearch mitochondrionsearch AMP-activated protein kinase complexsearch nucleussearch plasma membranesearch cell projectionsearch ciliumsearch nucleoplasmsearch sperm midpiecesearch
B (P00514) regulation of protein phosphorylationsearch cAMP-dependent protein kinase regulator activitysearch cAMP-dependent protein kinase complexsearch

Chain InterPro annotation
A Protein kinase domainsearch AGC-kinase, C-terminalsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B Cyclic nucleotide-binding domainsearch cAMP/cGMP-dependent protein kinasesearch cAMP-dependent protein kinase regulatory subunitsearch RmlC-like jelly roll foldsearch Cyclic nucleotide-binding, conserved sitesearch Cyclic nucleotide-binding-likesearch