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PDBe Entry: 2qbf view

Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF). This file contains the 30S subunit of the second 70S ribosome. The entire crystal structure contains two 70S ribosomes and is described in remark 400.
Summary
Header RIBOSOMEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 3.3 Å, R-factor: 27.5%, Free R-factor: 30.4%, Spacegroup: P 21 21 21
Released 25/09/2007, deposition: 16/06/2007, last revision: 22/09/2009
Authors Borovinskaya, M.A.search; Pai, R.D.search; Zhang, W.search; Schuwirth, B.-S.search; Holton, J.M.search; Hirokawa, G.search; Kaji, H.search; Kaji, A.search; Cate, J.H.D.search
Primary citation Structural basis for aminoglycoside inhibition of bacterial ribosome recycling.
NAT.STRUCT.MOL.BIOL.search vol:14, pag:727-732 (2007) [PubMed ID 17660832 ]search
Keywords RNA-protein complexsearch, RIBOSOMEsearch
Organism Escherichia coli 562search(C D E F G H I J K L M N O P Q R S T B A U)
UniProt 30S ribosomal protein S3 P0A7V3search (C)
30S ribosomal protein S4 P0A7V8search (D)
30S ribosomal protein S5 P0A7W1search (E)
30S ribosomal protein S6 [Contains: 30S ribosomal protein S6, fully modified isoform; 30S ribosomal protein S6, non-modified isoform] P02358search (F)
30S ribosomal protein S7 P02359search (G)
30S ribosomal protein S8 P0A7W7search (H)
30S ribosomal protein S9 P0A7X3search (I)
30S ribosomal protein S10 P0A7R5search (J)
30S ribosomal protein S11 P0A7R9search (K)
30S ribosomal protein S12 P0A7S3search (L)
30S ribosomal protein S13 P0A7S9search (M)
30S ribosomal protein S14 P0AG59search (N)
30S ribosomal protein S15 P0ADZ4search (O)
30S ribosomal protein S16 P0A7T3search (P)
30S ribosomal protein S17 P0AG63search (Q)
30S ribosomal protein S18 P0A7T7search (R)
30S ribosomal protein S19 P0A7U3search (S)
30S ribosomal protein S20 P0A7U7search (T)
30S ribosomal protein S2 P0A7V0search (B)
30S ribosomal protein S21 P68679search (U)
Solvent A, E, N, T
Related entries 2qbd, 2qbe, 2qbg, 2qbd, 2qbe, 2qbf, 2qbg
Polymers
Id Name Type UniProt Residues Observed
C 30S ribosomal protein S3 Protein P0A7V3 (RS3_ECOLI)search
232 88%
D 30S ribosomal protein S4 Protein P0A7V8 (RS4_ECOLI)search
205 100%
E 30S ribosomal protein S5 Protein P0A7W1 (RS5_ECOLI)search
166 90%
F 30S ribosomal protein S6 Protein P02358 (RS6_ECOLI)search
135 74%
G 30S ribosomal protein S7 Protein P02359 (RS7_ECOLI)search
178 85%
H 30S ribosomal protein S8 Protein P0A7W7 (RS8_ECOLI)search
129 100%
I 30S ribosomal protein S9 Protein P0A7X3 (RS9_ECOLI)search
129 98%
J 30S ribosomal protein S10 Protein P0A7R5 (RS10_ECOLI)search
103 95%
K 30S ribosomal protein S11 Protein P0A7R9 (RS11_ECOLI)search
128 91%
L 30S ribosomal protein S12 Protein P0A7S3 (RS12_ECOLI)search
123 100%
M 30S ribosomal protein S13 Protein P0A7S9 (RS13_ECOLI)search
117 96%
N 30S ribosomal protein S14 Protein P0AG59 (RS14_ECOLI)search
100 96%
O 30S ribosomal protein S15 Protein P0ADZ4 (RS15_ECOLI)search
89 98%
P 30S ribosomal protein S16 Protein P0A7T3 (RS16_ECOLI)search
82 97%
Q 30S ribosomal protein S17 Protein P0AG63 (RS17_ECOLI)search
83 97%
R 30S ribosomal protein S18 Protein P0A7T7 (RS18_ECOLI)search
74 74%
S 30S ribosomal protein S19 Protein P0A7U3 (RS19_ECOLI)search
91 87%
T 30S ribosomal protein S20 Protein P0A7U7 (RS20_ECOLI)search
86 98%
B 30S ribosomal protein S2 Protein P0A7V0 (RS2_ECOLI)search
240 90%
A 16S rRNA RNA
1542 99%
U 30S ribosomal protein S21 Protein P68679 (RS21_ECOLI)search
70 72%
Heterogens
Id Name Ligands
E, A MAGNESIUM ION MG search
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