› 2pz1

X-ray diffraction
2.25Å resolution

Crystal Structure of Auto-inhibited Asef

Released:
DOI: 10.2210/pdb2pz1/pdb
PDB entry 2pz1 coloured by chain, front view.
PDB entry 2pz1 coloured by chain, side view.
PDB entry 2pz1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression.
Mitin N, Betts L, Yohe ME, Der CJ, Sondek J, Rossman KL
Nat Struct Mol Biol 14 814-23 (2007)
PMID: 17704816

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 4 Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 53.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR80 (Residues: 170-632; Coverage: 67%)
Gene names: ARHGEF4, KIAA1112
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID, RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 100.418Å b: 79.883Å c: 67.407Å
α: 90° β: 122.99° γ: 90°
R-values:
R R work R free
0.221 0.217 0.279
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
9 more

6 mentions without citation

Release of autoinhibition of ASEF by APC leads to CDC42 activation and tumor suppression.
Mitin et al. (2007)
5 more