› 2pxz

X-ray diffraction
2.23Å resolution

E. coli phosphoenolpyruvate carboxykinase (PEPCK) complexed with ATP, Mg2+, Mn2+, carbon dioxide and oxaloacetate

Released:
DOI: 10.2210/pdb2pxz/pdb
PDB entry 2pxz coloured by chain, front view.
PDB entry 2pxz coloured by chain, side view.
PDB entry 2pxz coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Delbaere LTJ, Cotelesage JJH, Goldie H

Function and Biology Details

Reaction catalysed: 
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149561 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxykinase (ATP) Chain: X
Molecule details ›
Chain: X
Length: 540 amino acids
Theoretical weight: 59.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P22259 (Residues: 1-540; Coverage: 100%)
Gene names: JW3366, b3403, pck, pckA
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand MN 1 x MN
Ligand OAA 1 x OAA
Ligand ATP 1 x ATP
Ligand CO2 1 x CO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 124.556Å b: 94.302Å c: 46.354Å
α: 90° β: 95.54° γ: 90°
R-values:
R R work R free
0.176 0.173 0.234
Expression system: Escherichia coli K-12

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