› 2px3

X-ray diffraction
3.2Å resolution

Crystal structure of FlhF complexed with GTP/Mg(2+)

Released:
DOI: 10.2210/pdb2px3/pdb
PDB entry 2px3 coloured by chain, front view.
PDB entry 2px3 coloured by chain, side view.
PDB entry 2px3 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP.
Bange G, Petzold G, Wild K, Parlitz RO, Sinning I
Proc Natl Acad Sci U S A 104 13621-5 (2007)
PMID: 17699634

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169645 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flagellar biosynthesis protein FlhF Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.43 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01960 (Residues: 79-366; Coverage: 79%)
Gene names: BSU16400, flhF
Sequence domains: SRP54-type protein, GTPase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41212
Unit cell:
a: 80.965Å b: 80.965Å c: 126.137Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.31
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
10 more

4 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
3 more