› 2ptm

X-ray diffraction
1.93Å resolution

Structure and rearrangements in the carboxy-terminal region of SpIH channels

Released:
DOI: 10.2210/pdb2ptm/pdb
PDB entry 2ptm coloured by chain, front view.
PDB entry 2ptm coloured by chain, side view.
PDB entry 2ptm coloured by chain, top view.
Source organism: Strongylocentrotus purpuratus
Primary publication:
Structure and rearrangements in the carboxy-terminal region of SpIH channels.
Flynn GE, Black KD, Islas LD, Sankaran B, Zagotta WN
Structure 15 671-82 (2007)
PMID: 17562314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131244 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 198 amino acids
Theoretical weight: 23.29 KDa
Source organism: Strongylocentrotus purpuratus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O76977 (Residues: 470-665; Coverage: 26%)
Sequence domains: Cyclic nucleotide-binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NCO 1 x NCO
Ligand CMP 1 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P4212
Unit cell:
a: 92.354Å b: 92.354Å c: 63.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Hyperpolarization-activated cation channels: from genes to function.
Biel et al. (2009)
7 more

5 mentions without citation

Structural changes during HCN channel gating defined by high affinity metal bridges.
Kwan et al. (2012)
4 more