› 2poh

X-ray diffraction
2.1Å resolution

Structure of Phage P22 Tail Needle gp26

Released:
DOI: 10.2210/pdb2poh/pdb
PDB entry 2poh coloured by chain, front view.
PDB entry 2poh coloured by chain, side view.
PDB entry 2poh coloured by chain, top view.
Source organism: Salmonella phage P22-pbi
Primary publication:
Structure of phage P22 cell envelope–penetrating needle.
Olia AS, Casjens S, Cingolani G
Nat Struct Mol Biol 14 1221-6 (2007)
PMID: 18059287

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153043 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tail needle protein gp26 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 233 amino acids
Theoretical weight: 24.86 KDa
Source organism: Salmonella phage P22-pbi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35837 (Residues: 1-233; Coverage: 100%)
Gene name: 26
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 40.398Å b: 114.029Å c: 171.917Å
α: 90° β: 90.74° γ: 90°
R-values:
R R work R free
0.167 not available 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Bacterial contact-dependent delivery systems.
Hayes et al. (2010)
5 more

14 mentions without citation

'Let the phage do the work': using the phage P22 coat protein structures as a framework to understand its folding and assembly mutants.
Teschke et al. (2010)
13 more